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Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer

Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer
360,61 €
Artikelnummer: STRSPR-325B

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

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Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer

Details

Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer
Target: Alpha Synuclein
Purification: Ion-exchange Purified
Specificity: ~14.46 kDa
Nature: Recombinant
Expression system: E. coli
Amino Acid Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Conjugate: No tag
Biological Reactivity: 100 µM A53T alpha synuclein protein monomer (SPR-325) seeded with 10 nM A53T alpha synuclein protein PFF (SPR-326) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Protein Length: Full Length
Cellular Location: Cytoplasm, Membrane, Nucleus
Scientific Background: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease and increased rates of alpha synuclein fibrillization.
Reference: 1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230.5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.7. Polymeropoulos, M. H. (1998). Science. 276(5321), 2045–20478. Conway, K.E., et al. (1998). Nat Med. 4(11):1318-20

Zusatzinformation

Artikelnummer STRSPR-325B
Hersteller Stressmarq Biosciences
Herstellernummer SPR-325B
Verpackungseinheit 100 µg
Mengeneinheit FL
Reaktivität Human
Klonalität Keine Herstellerangabe
Wirt / Host Keine Herstellerangabe
Methode In Vitro Assay, In Vivo Assay, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), Western Blotting
Isotype Keine Herstellerangabe
Gene ID NCBI (externer Link)
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