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Human PAI-1 NBD labeled at the reactive center loop

Human PAI-1 NBD labeled at the reactive center loop
1.293,65 €
Artikelnummer: MOIHPAI-P9NBD-1

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

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Human PAI-1 NBD labeled at the reactive center loop, 1,0 mg

Details

P9-NBD PAI-1 was created by mutagenesis of the P9 serine residue (Ser338) on the reactive center loop to cysteine. This then provided a free thiol group for incorporation of N,N’-dimethyl-N-(acetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD), a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P9-NBD PAI-1 is enhanced 6-7 fold upon insertion of the reactive center loop into beta-sheet A following complex formation with proteinases, formation of the latent species, or cleavage by elastase. The incorporated probe is excited at 480 nm and displays a broad emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520 nm following reactive center loop insertion. The modified PAI-1 is nearly as active as wt PAI-1 and is more resistant to the spontaneous latency reaction making this an excellent tool for monitoring reaction rates of PAI-1 (1). P9-NBD PAI-1 has been utilized in a number of studies to determine the rates of loop insertion and SERPIN reaction mechanisms when reacted with various proteinases (1,2), inactivating antibodies (2) and conformational changes imposed by the binding of vitronectin (4).

Zusatzinformation

Artikelnummer MOIHPAI-P9NBD-1
Hersteller Molecular Innovations
Herstellernummer HPAI-P9NBD-1
Verpackungseinheit 1,0 mg
Mengeneinheit FL
Reaktivität Human
Klonalität Keine Herstellerangabe
Wirt / Host Keine Herstellerangabe
Methode Keine Herstellerangabe
Isotype Keine Herstellerangabe
Gene ID NCBI (externer Link)
Datenblatt Auf Anfrage
MSDS Download