Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells.
DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 10-101-CV is augmented with 4.5 g/L glucose and sodium pyruvate, and is supplemented with cellgro® glutagro™ supplement in place of L-glutamine for optimal performance.
Greatly improved stability over the L-glutamine media supplement.