UltraCULTURE™ Serum-free Medium is a complete, allpurpose medium designed for the cultivation of a wide variety of adherent and non-adherent mammalian cell types. UltraCULTURE™ Medium can be used to support fusion of cells during hybridoma formation, growth of monocyte, macrophage, epithelial, and fibroblastic cell lines.The medium is supplemented with recombinant human insulin, bovine transferrin, and a purified mixture of bovine serum proteins. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium can be supplemented with Cryoprotective Medium (Cat. No. 12-132A) to cryopreserve cells in a serum-free environment. UltraCULTURE™ Medium does not contain L-glutamine; please add 5 ml of 200 mM L-glutamine solution (Cat. Nos. 17-605 or 17-905) prior to use.The formulation for UltraCULTURE™ Medium has been submitted to the FDA as a Master File. Permission to crossreference the Master File may be obtained by contacting the Regulatory Affairs Department. Performance and Quality Tests – Turbidity may develop in UltraCULTURE™ Medium; experiments have determined that the turbidity will not alter the performance of the productAll BioWhittaker™ Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures.