The X-VIVO™ 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peri pheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2. X-VIVO™ 10 Media is available as a 1X liquid in two convenient formulations.X-VIVO™ 10, catalog number BE04-380Q contains L-glutamine, gentamicin, and phenol red. Catalog number BE04-743Q contains L-glutamine but does not contain gentamicin or phenol red. Catalog number BE02-055Q contains L-glutamine and recombinant transferrin but does not contain gentamicin or phenol red. The recombinant transferrin replaces native transferrin.All BioWhittaker™ Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures.