MEF 2 Nucleofector® Kit

The Nucleofector™ 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Mouse Embryonic Fibroblast Nucleofector™ Kit 2 is one of our two kits suited for efficient transfectiion of primary mouse embyonic fibroblasts (MEF). Due to significant diversity between different MEF clones, we have developed two Nucleofector™ Kits (1 and 2) and an easy-to-use Nucleofector™ Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone.

Content:
•2.25 mL Nucleofector™ Solution for Mouse Embryonic Fibroblasts
•0.5 mL Supplement 1
•30 µg pmaxGFP™ Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
•25 Aluminum cuvette (100 µL)
•25 Single use pipettes