hPB CD8+/CD45RA+, 5M cells

Leukapheresed Naïve CD8+ T cells are isolated using a two step isolation process. CD8+ T Cells are first negatively selected from MNC using an indirect immunomagnetic CD8+ labeling system. CD45RO microbeads are then used to deplete the CD45RO+ population, leaving a highly enriched CD4+ CD45RA+ Naïve T Cell population. Cells are cryopreserved in Lonza Cryo Media and collected in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.