CloneID: VHHCD8
Antigen Long Description: The original antibody was generated by immunizing a llama with CD8, followed by antibody library construction. The antibody was isolated by biopanning against CD8.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a chimeric antibody, developed from the original camelid VHH antibody, specifically engineered for enhanced compatibility with existing reagents, assays, and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P01731
Specificity Statement: The antibody is specific for CD8.
Application Notes (Clone): The binding affinity of the original format of the antibody to CD8 was measured by surface plasmon resonance (Kd= 0.9 nM). FACS analysis of splenocytes and lymph nodes gated on CD45+CD19−CD3+ cells, confirming that the original format of the antibody stained CD8+ cells. The antibody competed with a commercial anti-CD8α antibody, showing it bound to the CD8α chain. 89Zr-PEGylated antibody was used for PET imaging on mice, to track the presence of intratumoral CD8+ T cells (Rashidian et al., 2017; PMID: 28666979).
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