CloneID: ScFv5
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was isolated from a human scFv phage-displayed library by panning against SpuE.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human scFv antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q9I6J0
Specificity Statement: The antibody binds specifically to SpuE. In particular, it interacts with the hinge region that connects the two domains of SpuE.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody conjugated to a membrane penetrating peptide (MPP) could inhibit the secretion of ExoS from P. aeruginosa. The antibody conjugated to MPP was able to inhibit the cytotoxic effects of SpuE in the human epithelial cell line A549. The antibody conjugated to MPP showed a protective effect on C. elegans from P. aeruginosa infection. The crystal structure of the antibody in complex with SpuE-spermidine was determined. The dissociation constant (KD) of SpuE to spermidine and of SpuE to spermidine in the presence of the antibody was measured by isothermal titration calorimetry (Kd = 2.7 nM and 29.8 nM, respectively) (Zhang et al., 2019; PMID: 31682834).
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