Target: Aquaporin 4
Conjugate: ATTO 390
Product Type: Polyclonal
Immunogen: Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4
Swiss-Prot: P47863
Purification: Protein A Purified
Storage Buffer: 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS
Concentration: 1 mg/ml
Specificity: Detects ~35kDa. May detect larger glycosylated bands ~50kDa.
Cellular Localization: Membrane
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica ( 5, 6).
References: 1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.3. Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415.4. Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913.5. Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473-477.6. Bradl M., Lassmann D.H. (2008) Int. MS Journal/MS Forum. 15(3): 75-78.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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