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Cytokeratin 19
Cytokeratin 19, IgG1, Host: Mouse, Monoclonal, Clone: RCK108
Artikelnummer
BILCK119
Verpackungseinheit
1 ml
Hersteller
BioLogo
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Clone: RCK108.
Background: Cytokeratin 19 is found in most simple epithelia and non keratinised squamous epithelia with predominant focal reactivity. RCK108 stains practically all epithelial tumours, especially adenocarcinoma. It is negative in hepatocellular carcinoma (4). Most medullary, weakly differentiated ductal carcinoma are also CK19-negative (3). Cytokeratins are a group of water insoluble filament proteins, which are constituents of the cytoskeleton of epidermal cells and other epithelial cells. By gel electrophoretic analysis up to now 20 different cytokeratins have been characterized. They have been divided into basic and acid subfamilies and may also be distinguished by their molecular weights and their tissue distribution. The most frequently used nomenclature has been published by R. Moll et al. (1982). Antibody RCK108 reacts with a 40 kDa keratin (Cytokeratin 19) in immunoblot.
Positive Control: Glandular epithelia.
Immunogen: Cell line T24.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
References: 1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Smedts F., Ramaekers F., Robben H., et al. (1990) Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J. Pathol. 136,3; 657-668. 3. Dalal P. and Shousha S. (1995) Keratin 19 in paraffin sections of medullary carcinoma and other benign and malignant breast lesions. Mod. Pathol. 8(4); 413-416. 4. Kwaspen F.H., Smedts F.M., Broos A., Bulten H., Dedie W.M., and Raemaekers F.C. (1997) Reproducible and highly sensitive detection of the broad spectrum epithelium marker keratin 19 in routine cancer diagnosis. Histopathol. 31(6); 503-516.
UniProt: P08727.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Background: Cytokeratin 19 is found in most simple epithelia and non keratinised squamous epithelia with predominant focal reactivity. RCK108 stains practically all epithelial tumours, especially adenocarcinoma. It is negative in hepatocellular carcinoma (4). Most medullary, weakly differentiated ductal carcinoma are also CK19-negative (3). Cytokeratins are a group of water insoluble filament proteins, which are constituents of the cytoskeleton of epidermal cells and other epithelial cells. By gel electrophoretic analysis up to now 20 different cytokeratins have been characterized. They have been divided into basic and acid subfamilies and may also be distinguished by their molecular weights and their tissue distribution. The most frequently used nomenclature has been published by R. Moll et al. (1982). Antibody RCK108 reacts with a 40 kDa keratin (Cytokeratin 19) in immunoblot.
Positive Control: Glandular epithelia.
Immunogen: Cell line T24.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
References: 1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Smedts F., Ramaekers F., Robben H., et al. (1990) Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J. Pathol. 136,3; 657-668. 3. Dalal P. and Shousha S. (1995) Keratin 19 in paraffin sections of medullary carcinoma and other benign and malignant breast lesions. Mod. Pathol. 8(4); 413-416. 4. Kwaspen F.H., Smedts F.M., Broos A., Bulten H., Dedie W.M., and Raemaekers F.C. (1997) Reproducible and highly sensitive detection of the broad spectrum epithelium marker keratin 19 in routine cancer diagnosis. Histopathol. 31(6); 503-516.
UniProt: P08727.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
| Artikelnummer | BILCK119 |
|---|---|
| Hersteller | BioLogo |
| Hersteller Artikelnummer | CK119 |
| Verpackungseinheit | 1 ml |
| Mengeneinheit | STK |
| Reaktivität | Human, Zebrafish |
| Klonalität | Monoclonal |
| Methode | Immunohistochemistry (frozen), Immunohistochemistry (paraffin) |
| Isotyp | IgG1 |
| Wirt | Mouse |
| Produktinformation (PDF) | Download |
| MSDS (PDF) | Download |
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