Background: GPR75 is a member of the G protein-coupled receptor family and is a novel target for the clinical treatment of obesity. Human GPR75 haploinsufficiency exhibits a striking phenotype of low body fat, and GPR75 knockout mice are hypophagic and thin, improving glucose tolerance and insulin sensitivity. To enhance the expression level of GPR75, twenty-four amino acids from the β2AR receptor (MGQPGNGSAFLLAPNRSHAPDHDV) were used to replace the original residues1-31 in a modified version of the GPR75 receptor. Furthermore, the BRIL sequence was used to replace the original ICL3 loop (237-306) to enhance receptor stability. BRIL is thermostabilized apocytochrome b562 with mutations M7W, H102I, R106L, PDB ID 1M6T.
Description: Human β2AR-GPR75-BRIL full length protein-synthetic nanodisc
Molecular Weight: The human full length β2AR-GPR75-BRIL protein has a MW of 59.4 kDa
Protein Families: Druggable Genome, GPCR, Transmembrane
Protein Pathways: N/A
Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Tag: C-Flag Tag
Expression Host: HEK293
Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments.
Target: GPR75
Uniprot ID: O95800
Usage: Research use only
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