Clone: R013
Specificity: Human IL-5. Has cross-reactivity with mouse IL5 in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Immunogen: Recombinant Human IL-5/IL5/Interleukin 5 protein
Gene: IL5
Description: IL5 antibody LS-C819032 is an unconjugated rabbit monoclonal antibody to human IL5. Validated for Block, ELISA and Neut.
Usage: ELISA(Det): This antibody will detect Human IL-5/IL5 / Interleukin 5 in ELISA pair set. Block: In a functional ELISA which immobilized Human IL5 at 0.4 ug/ml (100 µL/well) in the plate, the Rabbit anti-Human IL5 Monoclonal Antibody can block the binding of 2 ug/ml Human IL5R to human IL5, the EC50 is 0.23 ug/ml. Neutralization: The neutralization activity of IL5 neutralizing antibody is measured by its ability to neutralize IL5-induced proliferation in the TF-1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 2-8 ng/mL in the presence of 1. 25 ng/mL Human IL5. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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