English
Mouse anti Human CD95
Mouse anti Human CD95, Monoclonal, IgG1, Clone: B-R18
Artikelnummer
EXAX2785M
Verpackungseinheit
100 µg
Hersteller
Exalpha Biologicals Inc
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Clone: B-R18.
Background: CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
Positive Control: This antibody clone B-R18 specifically recognizes CD95. B-R18 reacts with peripheral lymphocytes and especially with peripheral monocytes. It stains human B cell lines like pre-B cells, EBV cells, Burkitt cells and plasmacytoma cells. It also binds to human T cell lines, myeloid cell lines, hepatocyte carcinoma and endothelial cells.
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with purified recombinant Fas antigen and fused with X63/Ag.8653 mouse myeloma cells.
Purification Method: Protein A/G Chromatography.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1) The 55-kD tumor necrosis factor receptor and CD95 independently signal murine hepatocyte apoptosis and subsequent liver failure. Author : Leist M; Gantner F; K ̈unstle G; Bohlinger I; Tiegs G; Bluethmann H; Wendel A Source : Mol Med, 2(1):109-24 1996 Jan
2) Regulation of CD95 ligand expression: a key element in immune regulation? Author : Brunner T; Yoo NJ; Griffith TS; Ferguson TA; Green DR Source : Behring Inst Mitt, (97):161-74 1996 Oct
3)Expression of CD95 antigen and Bcl-2 protein in non-Hodgkin's lymphomas and Hodgkin's disease [published erratum appears in Am J Pathol 1996 Jul;149(1):346] Author : Nguyen PL; Harris NL; Ritz J; Robertson MJ Source : Am J Pathol, 148(3):847-53 1996 Mar
4) Relation of oxidative stress and glutathione synthesis to CD95(Fas/APO-1)-mediated apoptosis of adult T cell leukemia cells. Author : Kohno T; Yamada Y; Hata T; Mori H; Yamamura M; Tomonaga M; Urata Y; Goto S; Kondo T Source : J Immunol, 156(12):4722-8 1996 Jun 15
5) Clonal deletion of major histocompatibility complex class I-restricted CD4+CD8+ thymocytes in vitro is independent of the CD95 (APO-1/Fas) ligand. Author : M ̈uller KP; Mariani SM; Matiba B; Kyewski B; Krammer PH Source : Eur J Immunol, 25(10):2996-9 1995 Oct
6) Activation of the CD95 system increases with disease progression in human immunodeficiency virus type 1-infected children and adolescents. Author : B ̈ohler T; B ̈aumler C; Herr I; Groll A; Kurz M; Debatin KM Source : Pediatr Infect Dis J, 16(8):754-9 1997 Aug
7) Fc gammaRIII-mediated regulation of hematopoiesis in murine bone marrow cells by interleukin-3 and CD95 (Fas/Apo-1). Author : Yoshikawa H; Sakihama T; Nakajima Y; Tasaka K Source : Blood, 90(5):1911-9 1997 Sep 1
8) Human autoreactive and foreign antigen-specific T cells resist apoptosis induced by soluble recombinant CD95 ligand. Author : Zipp F; Martin R; Lichtenfels R; Roth W; Dichgans J; Krammer PH; Weller M Source : J Immunol, 159(5):2108-15 1997 Sep 1
9) The CD95 (APO-1/Fas) system mediates drug-induced apoptosis in neuroblastoma cells. Author : Fulda S; Sieverts H; Friesen C; Herr I; Debatin KM Research Center, Heidelberg. Source : Cancer Res, 57(17):3823-9 1997 Sep 1
10) Differential CD95 expression and function in T and B lineage acute lymphoblastic leukemia cells. Author : Karawajew L; Wuchter C; Ruppert V; Drexler H; Gruss HJ; D ̈orken B; Ludwig W. D. Source : Leukemia, 11(8):1245-52 1997 Aug.
UniProt: P25445.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Background: CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
Positive Control: This antibody clone B-R18 specifically recognizes CD95. B-R18 reacts with peripheral lymphocytes and especially with peripheral monocytes. It stains human B cell lines like pre-B cells, EBV cells, Burkitt cells and plasmacytoma cells. It also binds to human T cell lines, myeloid cell lines, hepatocyte carcinoma and endothelial cells.
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with purified recombinant Fas antigen and fused with X63/Ag.8653 mouse myeloma cells.
Purification Method: Protein A/G Chromatography.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1) The 55-kD tumor necrosis factor receptor and CD95 independently signal murine hepatocyte apoptosis and subsequent liver failure. Author : Leist M; Gantner F; K ̈unstle G; Bohlinger I; Tiegs G; Bluethmann H; Wendel A Source : Mol Med, 2(1):109-24 1996 Jan
2) Regulation of CD95 ligand expression: a key element in immune regulation? Author : Brunner T; Yoo NJ; Griffith TS; Ferguson TA; Green DR Source : Behring Inst Mitt, (97):161-74 1996 Oct
3)Expression of CD95 antigen and Bcl-2 protein in non-Hodgkin's lymphomas and Hodgkin's disease [published erratum appears in Am J Pathol 1996 Jul;149(1):346] Author : Nguyen PL; Harris NL; Ritz J; Robertson MJ Source : Am J Pathol, 148(3):847-53 1996 Mar
4) Relation of oxidative stress and glutathione synthesis to CD95(Fas/APO-1)-mediated apoptosis of adult T cell leukemia cells. Author : Kohno T; Yamada Y; Hata T; Mori H; Yamamura M; Tomonaga M; Urata Y; Goto S; Kondo T Source : J Immunol, 156(12):4722-8 1996 Jun 15
5) Clonal deletion of major histocompatibility complex class I-restricted CD4+CD8+ thymocytes in vitro is independent of the CD95 (APO-1/Fas) ligand. Author : M ̈uller KP; Mariani SM; Matiba B; Kyewski B; Krammer PH Source : Eur J Immunol, 25(10):2996-9 1995 Oct
6) Activation of the CD95 system increases with disease progression in human immunodeficiency virus type 1-infected children and adolescents. Author : B ̈ohler T; B ̈aumler C; Herr I; Groll A; Kurz M; Debatin KM Source : Pediatr Infect Dis J, 16(8):754-9 1997 Aug
7) Fc gammaRIII-mediated regulation of hematopoiesis in murine bone marrow cells by interleukin-3 and CD95 (Fas/Apo-1). Author : Yoshikawa H; Sakihama T; Nakajima Y; Tasaka K Source : Blood, 90(5):1911-9 1997 Sep 1
8) Human autoreactive and foreign antigen-specific T cells resist apoptosis induced by soluble recombinant CD95 ligand. Author : Zipp F; Martin R; Lichtenfels R; Roth W; Dichgans J; Krammer PH; Weller M Source : J Immunol, 159(5):2108-15 1997 Sep 1
9) The CD95 (APO-1/Fas) system mediates drug-induced apoptosis in neuroblastoma cells. Author : Fulda S; Sieverts H; Friesen C; Herr I; Debatin KM Research Center, Heidelberg. Source : Cancer Res, 57(17):3823-9 1997 Sep 1
10) Differential CD95 expression and function in T and B lineage acute lymphoblastic leukemia cells. Author : Karawajew L; Wuchter C; Ruppert V; Drexler H; Gruss HJ; D ̈orken B; Ludwig W. D. Source : Leukemia, 11(8):1245-52 1997 Aug.
UniProt: P25445.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
