Target: NKCC2
Conjugate: ATTO 390
Product Type: Polyclonal
Immunogen: Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
Swiss-Prot: Q5PQV1
Purification: Protein A Purified
Storage Buffer: 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS
Concentration: 1 mg/ml
Specificity: Detects ~160kDa.
Cellular Localization: Membrane
Scientific Background: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
References: 1. Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971.2. Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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