Recombinant Human MeCP2 protein(His tag)

Protein Tag: N-His

Uniprot: P51608

Accession: P51608

Background: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

Bio Acitivity: Not validated for activity

Sequence: Met1-Lys177

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.

Calculated MW: 19.0 kDa

Observed MW: 32 kDa

• Artikelnummer: ELSPDEH100777-500
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: PDEH100777-500
• Verpackungseinheit: 500μg
• Mengeneinheit: STK
• Reaktivität: Human
• Wirt: Escherichia Coli