Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Target Synonym: NAD-dependent deacetylase sirtuin-3;SIR2-like protein 3;SIR2L3
Protein Tag: N-Sumo
Background: SIRT3 is a nicotinamide adenine dinucleotide (NAD(+))-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Sirtuin 3 (SIRT3) mediates cellular resistance toward various forms of stress. sirtuin 3 (SIRT3), the mitochondrial NAD(+)-dependent deacetylase, may regulate mitochondrial function and biosynthetic pathways such as glucose and fatty acid metabolism and the tricarboxylic acid (TCA) cycle, oxidative stress, and apoptosis by reversible protein lysine deacetylation. SIRT3 regulates glucose and lipid metabolism and maintains myocardial ATP levels, which protects the heart from metabolic disturbances. SIRT3 can also protect cardiomyocytes from oxidative stress-mediated cell damage and block the development of cardiac hypertrophy. Recent reports show that SIRT3 is involved in the protection of several heart diseases.
Bio Activity: Not validated for activity
Sequence: Gly202-Lys399
Purity: > 95% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Calculated MW: 34.5 kDa
Observed MW: 36 kDa
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