Recombinant Human UCHL1 protein (GST tag)

Protein Tag: N-GST

Uniprot: P09936

Accession: P09936

Background: Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.

Bio Acitivity: Not validated for activity

Sequence: Met1-Cys220

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.

Calculated MW: 49.1 kDa

Observed MW: 30 kDa

• Artikelnummer: ELSPDEH100784-500
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: PDEH100784-500
• Verpackungseinheit: 500μg
• Mengeneinheit: STK
• Reaktivität: Human
• Wirt: Escherichia Coli