Target Synonym: SAHH
Tested Sample Types: Animal tissue;Cell
Assay Time: 1 h
Detection Type: Colorimetric method
Sensitivity: 1.34 U/L
Detection Range: 1.34-37.14 U/L
Test Principle: In mammalian cells, S-adenosine homocysteine hydrolase (SAHH) is the only hydrolase involved in the hydrolysis of S-adenosine homocysteine (SAH) to homocysteine (Hcy). Excessive SAHH activity can hydrolyze SAH to generate excessive Hcy, thereby causing geriatric diseases such as Alzheimer's disease, hypercysteinemia, and coronary heart disease. Inhibiting SAHH activity can effectively alleviate the hydrolysis of SAH.
The detection principle of this kit: The substrate is hydrolyzed by SAHH, and the product reacts with the chromogenic agent at room temperature to form a yellow colored product, which has the maximum absorption peak at 410 nm. The activity of SAHH can be calculated by detecting the change in absorbance at 410 nm.
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