Product Description: Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin. Streptavidin-biotin binding is one of the strongest known noncovalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's affinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Phycoerythrin (PE) is a red-pigmented protein found in cyanobacteria and red algae. Phycoerythrin absorbs light blue-green/yellow light and emits lightly orange/yellow light.
Application Note: IHC-Fr: 1:100-1:250. FCM: 1:100 - 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Form: Liquid
Buffer (with preservative): 20 mM K3PO4, 150 mM NaCl, 1% BSA, 0.01% Sodium Azide.
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Purification: Purified streptavidin (PE) electrophoretically
Conjugation: PE
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