Target: Synaptotagmin 7
Conjugate: RPE
Product Type: Monoclonal
Clone Number: N275/14 (Formerly sold as S275-14)
Immunogen: Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
Swiss-Prot: Q9R0N7
Purification: Protein G Purified
Storage Buffer: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration: 1 mg/ml
Specificity: Detects ~45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.
Cellular Localization: Cytoplasmic Vesicle
Scientific Background: Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
References: 1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815.2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Deutsch
English
