Shelf life (days): 730
Info: Cayman’s T7 mRNA Transcription Kit (Cap-1; mo5U) is optimized for the efficient synthesis of high-quality mRNA from a linear DNA template containing a T7 promoter sequence. Using T7 RNA polymerase, this kit enables the production of mRNA with high yield in vitro, which is suitable for a variety of downstream applications. The kit includes all essential reagents, including Cap-1, a modified 5’-cap analog, and 5-methoxy-UTP (mo5U). The cap facilitates efficient translation initiation, protects against exonuclease-mediated degradation, and improves the stability of the mRNA when delivered via lipid nanoparticles (LNPs) or other formulations. mo5U inhibits RNA-mediated innate immune activation. Each kit contains sufficient reagents for 25 reactions (20 µl per reaction). Each reaction can yield up to 100 µg of RNA.
Formula Weight: 0