Tau-441 (2N4R) Wild-Type Pre-formed Fibrils (Baculovirus/Sf9)

Human Recombinant Tau-441 (2N4R) Wild-Type Pre-formed Fibrils (Baculovirus/Sf9)

Target: Tau

Nature: Recombinant

Swiss-Prot: P10636-8

Expression System: Baculovirus (Sf9)

Protein Length: Full Length (1-441 aa)

Amino Acid Sequence: MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

Purification: Ion-exchange Purified

Purity: >95%

Storage Buffer: 1X PB pH7.4

Protein Size: 45.84 kDa

Conjugate: No Tag

Scientific Background: Brain-specific tau isoforms vary in the number of N-terminal inserts and C- terminal repeat domains due to alternative splicing of exons; the 2N4R isoform of tau is expressed in adult brain yet is absent from the fetal brain (1). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as Alzheimer’s Disease (AD) are characterized by neurofibrillary tangles containing hyper-phosphorylated tau fibrils (2). Hyper-phosphorylated tau can be generated via expression in the Sf9/Baculovirus system, with up to 20 sites confirmed by mass spectrometry and Western Blots with phospho-specific antibodies(3). Sf9/Baculovirus-expressed Tau 2N4R Pre-Formed Fibrils are generated without heparin.

References: 1. Goedert et al. 1989. Multiple Isoforms of Human Microtubule-associated Protein Tau: Sequences and Localization in Neurofibrilary Tangles of Alzheimer’s Disease. Neuron. doi: 10.1016/0896-6273(89)90210-9. 2. Iqbal K., Liu F., and Gong C.X. 2016. Tau and neurodegenerative disease: The story so far. Nat. Rev. Neurol. DOI: 10.1038/nrneurol.2015.225 3. Tepper et al. 2014. Oligomer Formation of Tau Protein Hyperphophorylated in Cells. The Journal of Biological Chemistry, DOI 10.1074/jbc.M114.611368

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

• Artikelnummer: STRSPR-498B
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SPR-498B
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human
• Methode: Western Blotting, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), In Vitro Assay