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Application: Ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Assay Conditions: Reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2.
Description: Ultra-pure recombinant form of Serratia marcescens extracellular endonuclease. Nonspecific endonuclease, hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length. MW = 27 kDa (homodimer). Expressed in E. coli. No detectable protease activity.TurboNuclease can be used to
• reduce viscosity of cell lysates
• reduce back pressure of column loading
• remove nucleic acid contamination from sample preparations
• reduce nucleic acid contamination of Ni column purification
• reduce smearing in SDS-PAGE when used with 10%SDS or Gel Loading Dye
• make whole cell lysates
• reduce or prevent clumping of concentrated cells and thawed cells
• replace crude DNase I in many applications
Endotoxin Level: Total endotoxin level is < 0.25 EU/1,000 units of TurboNuclease as determined by the LAL Gel-Clot Assay. No protease activity was detected.
Purity: ≥99%
Storage Stability: >6 months at -20°C. Retains >80% activity after storage at RT for over 65 hours.
Supplied As: 250 units/µl in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% glycerol
Unit Definition: One unit converts 1 OD260 of salmon sperm DNA into acid soluble nucleotides in 30 minutes at 37°C
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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