Conjugate: APC
Product Type: Monoclonal
Clone Number: 10A8
Immunogen: Full length recombinant human VSP35
Swiss-Prot: Q96QK1
Purification: Protein G Purified
Storage Buffer: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration: 1 mg/ml
Specificity: Detects ~92 kDa.
Cellular Localization: Endosome / Lysosome / Vesicles / Cytoplasm / Membrane
Tissue Specificity: Ubiquitous. Highly expressed in brain, colon, heart, kidney, ovary, placenta, skeletal muscle, small intestine, testis, thymus.
Scientific Background: Vacuolar Protein Sorter 35 (VPS35) is a core component of the retromer complex, a critical intracellular trafficking system responsible for the endosome-to-Golgi retrieval of membrane proteins. This pathway is essential for maintaining protein homeostasis, synaptic function, and neuronal survival. Mutations in VPS35—most notably the D620N variant—have been directly linked to familial and sporadic forms of Parkinson’s disease (PD). These mutations impair retromer function, leading to disrupted recycling of key neuronal receptors and transporters. Dysfunctional VPS35 also contributes to mitochondrial fragmentation, oxidative stress, and impaired autophagy—cellular processes that are central to the pathogenesis of neurodegenerative diseases. Beyond Parkinson’s, VPS35 dysfunction has been implicated in Alzheimer’s disease and other proteinopathies, where it affects the trafficking of amyloid precursor protein (APP) and tau. Its role in regulating endosomal sorting and mitochondrial dynamics positions VPS35 as a critical node in the cellular pathways that maintain neuronal integrity. As a result, VPS35 is emerging as a promising therapeutic target in neurodegenerative disease research. Restoring or enhancing retromer function may offer novel strategies to counteract protein misfolding, synaptic loss, and neuroinflammation.
References: 1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167 2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175 3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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