4-HNE (4-Hydroxynonenal) ELISA Kit

Usage: This ELISA kit applies to the in vitro quantitative determination of 4-HNE concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Type: Competitive-ELISA

Assay Time: 2 h 30 min

Detection Type: Colormetric

Sensitivity: 0.38 ng/mL

Detection Range: 0.63-40 ng/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 4-HNE. During the reaction, Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Universal 4-HNE in samples.No significant cross-reactivity or interference between Universal 4-HNE and analogues was observed

Storage: 2-8℃/-20℃,12 months

Citation Link 1: https://www.sciencedirect.com/science/article/pii/S1385894722044709

Citation Link 2: https://www.sciencedirect.com/science/article/pii/S014296122200360X

Citation Link 3: https://www.sciencedirect.com/science/article/pii/S2213231721002895

• Artikelnummer: ELSE-EL-0128-96
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: E-EL-0128-96
• Verpackungseinheit: 96 T
• Mengeneinheit: PAK
• Methode: ELISA