Shelf life (days): 1460.0
Formulation: A crystalline solid
Formal Name: 5-fluoro-1,2,3,6-tetrahydro-2,6-dioxo-4-pyrimidinecarboxylic acid, monohydrate
Purity: ≥95%
Formula Markup: C5H3FN2O4 / H2O
Formula Weight: 192.1
CAS Number: 220141-70-8
Notes: 5-fluoro Orotic acid (5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs.{28301} Yeast with an active URA3 gene, which encodes orotidine-5'-monophosphate decarboxylase, converts 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant strains of yeast that carry a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil.