Application Note: WB: 1:10,000-1:100,000. ICC/IF: 1:200-1:500. ELISA: 1:10,000-1:100,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity/Sensitivity: This antibody does not recognize all N-terminal 6-His tagged proteins.
Form: Liquid
Buffer (with preservative): PBS, 0.2% BSA, 0.1% ProClin 400.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen: 6-His (HHHHHH) conjugated to KLH.
Purification: Purified by antigen-affinity chromatography
Conjugation: HRP