Abl Kinase, His-tag Recombinant

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Encompassing Amino Acids: 248-531

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed in Kinase buffer containing 0.2 mM DTT using Abltide substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).

Description: Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1) (GenBank Accession No. NM_007313), a.a. 248-531, with N-terminal His-tag, MW = 34.8 kDa, expressed in a Baculovirus-infected Sf9 cell expression system

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 200 mM imidazole, and 20% glycerol.

Genbank: NM_00731

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P00519

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Cao, C., et al. 2003. J. Biol. Chem. 278(15):12961-7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3):356-364.Application Reference:LASP1 is a novel BCR-ABL substrate and a phosphorylation-dependent binding partner of CRKL in chronic myeloid leukemia (2014)

• Artikelnummer: BPS40411
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40411
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect