ACC2, His-Tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: A 25 µl ACC2 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl₂, 2 mM potassium citrate, 1 mM DTT, 100 µM ATP, 12 mM NaHCO₃ and 20 µM acetyl-CoA at 30°C for 45 min. ATP reduction is detected using ADP-Glo® Luminescent Assay Platform (Promega).

Description: Human acetyl-Coenzyme A carboxylase alpha transcript variant 2 (ACC2), GenBank Accession No. NM_001093, full length, with C-terminal His-tag, MW=277 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.

Genbank: NM_001093

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: O00763

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Locke G.A. et al., Arch. Biochem. Biophys. 475 (1), 72-79 (2008).
2. Abu-Elheiga L. et al., Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1444-1449 (2000).Application Reference(s):
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

• Artikelnummer: BPS50201
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50201
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect