Target: Tau (K18) P301L Mutant
Nature: Recombinant
Swiss-Prot: P10636
Biological Activity: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when tau PFFs are combined with tau monomers.
Expression System: E. coli
Protein Length: Partial
Amino Acid Sequence: SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
Purification: Ion-exchange Purified
Purity: >95%
Storage Buffer: 10 mM HEPES, 100 mM NaCl pH 7.4
Protein Size: ~15.1 kDa
Conjugate: No tag
Cellular Localization: Cytoplasm / Axolemma / Axolemma Plasma Membrane / Axon / Cell Body / Cell membrane / Cytoplasmic Ribonucleoprotein Granule / Cytoplasmic Side / Cytoskeleton / Cytosol / Dendrite / Growth cone / Microtubule / Microtubule Associated Complex / Neurofibrillary Tangle / Neuronal Cell Body / Nuclear Periphery / Nuclear Speck / Nucleus / Peripheral membrane protein / Plasma Membrane / Tubulin Complex
Scientific Background: Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).
References: 1. www.alz.org/alzheimers-dementia/facts-figures2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18.5. Guo, J. and Lee, M.Y. (2013). FEBS Lett. 587(6): 717-723.6. Alberici, A. et al. (2004). J Neurol Neurosurg Psychiatry. 75:1607-1610.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
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