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AKT2, Active, His-tag Recombinant
AKT2, Active, His-tag Recombinant
Artikelnummer
BPS40011
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 6, Invitrogen) at 30 °C for 1 hour.
Description: Recombinant human AKT2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=60.2 kDa, was expressed in Sf9 insect cells via a baculovirus expression system. The purified AKT2 is activated in vitro using PDK1 enzyme.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 20% glycerol, and 3 mM DTT.
Genbank: NM_001626
Host Cell Line: Sf9 cells
Purification: Purified by affinity chromatography
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P31751
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Alessi et al., 1998. Current Biology 8: 69.
2. Cohen et al., 1997. FEBS Lett. 410: 3.
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 6, Invitrogen) at 30 °C for 1 hour.
Description: Recombinant human AKT2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=60.2 kDa, was expressed in Sf9 insect cells via a baculovirus expression system. The purified AKT2 is activated in vitro using PDK1 enzyme.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 20% glycerol, and 3 mM DTT.
Genbank: NM_001626
Host Cell Line: Sf9 cells
Purification: Purified by affinity chromatography
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P31751
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Alessi et al., 1998. Current Biology 8: 69.
2. Cohen et al., 1997. FEBS Lett. 410: 3.
| Artikelnummer | BPS40011 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40011 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
