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Protein Tag: N-His
Uniprot: P08758;C5MKY7
Accession: P08758&C5MKY7
Background: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.annexin A5,calcium and phospholipid binding protein,endonexin 2,placental protein 4,anchorin CII collagen receptor of chondrocytes,predominantly expressed in major constituent of growth plate chondrocytes,down-regulated in adult articular cartilage.EGFP can be used to detect apoptosis by flow cytometry or fluorescence microscopy.
Bio Acitivity: Not validated for activity
Sequence: Annexin V(1Met-320Asp)-(GGGGS)4-EGFP(1Met-239Lys)
Purity: > 90% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.
Calculated MW: 63.8 kDa
Observed MW: 62-70 kDa
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