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Protein Tag: C-His
Uniprot: Q12794
Accession: Q12794
Background: Human hyaluronidases (HYALs) are a group of five endo‑ beta ‑N‑acetyl‑hexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20). While HYAL1, HYAL2 and SPAM1 are endo‑ beta ‑N‑acetyl‑glucosaminidase that are mainly active on hyaluronan with little activity on chondroitin sulfate, HYAL4 is mainly active on chondroitin sulfate (type C and D in particular) (6). Hyaluronan and chondroitin sulfate are abundant extracellular matrix components that have numerous biological functions. The backbone of chondroitin sulfate composed of repeating units of ‑4GlcA beta 1‑3GalNAc beta 1‑ most closely resembles to that of hyaluronan composed of repeating units of ‑4GlcA beta 1‑3GlcNAc beta 1‑, which may partially explain the overlapping substrate specificity of most hyaluronidases. HYAL1 is a lysosomal hyaluronidase with optimal activity around pH 4.0. It is highly expressed in the liver, kidney and heart, and is the predominant hyaluronidase found in plasma. Defects in HYAL1 are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. Surprisingly, HYAL1 has been reported both as a tumor promoter and suppressor. The enzymatic activity of recombinant human HYAL1 was measured using 35S‑labeled hyaluronan as substrate.
Bio Acitivity: Not validated for activity
Sequence: Met1-Trp435
Purity: > 95% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Endotoxin: < 1.0 EU/mg of the protein as determined by the LAL method
Calculated MW: 47.7 kDa
Observed MW: 50-60 kDa
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