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Protein Tag: N-His
Uniprot: P05784
Accession: P05784-1
Background: Cytokeratin 18 (CK18) has a molecular weight of 45 KDa, which is a low molecular weight type A cytokeratin. It is a marker of simple epithelium, including glandular epithelium. The stratified squamous epithelium is generally negative. CK18 is mainly used in the diagnosis of adenocarcinoma.
Bio Acitivity: Not validated for activity
Sequence: Ser2-Asp231
Purity: > 85% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.
Calculated MW: 25.5 kDa
Observed MW: 27.78 kDa