CloneID: B1H12
Antigen Long Description: This antibody was raised by immunising α1,3galactosyltransferase knock-out mice, which lack α-gal epitopes. Mice were immunised with rabbit red cell membranes, on which α-gal is the major carbohydrate epitope.
Origin Pub PMID: 11090880
Buffer Composition: PBS only.
Specificity Statement: This antibody is specific for α-gal epitopes (Galα1–3Galβ1–4GlcNAc-R) on glycoprotein and glycolipid molecules.
Application Notes (Clone): This antibody binds to glycoprotein and glycolipid molecules expressing α-gal epitopes, including α-gal-BSA, in ELISA analysis (Chen et al., 2005). It does not cross-react with any glycoproteins lacking this epitope. This antibody binds to the α-gal epitopes on the hybridoma cells producing the antibody, so can mediate complement mediated autolysis in the presence of rabbit serum (Chen et al., 2000).
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