Anti-A33 (huA33)

Anti-A33 [huA33], Recombinant, IgG1 kappa, Human

CloneID: huA33

Heavy Chain modification: Fc Silent™

Antigen Long Description: hA33 was generated by humanization of mouse A33 IgG2a-κ by CDR-grafting, and expressed in NSO cells. The mouse A33 antibody was prepared by immunizing BALB/c mice with AsPC-1 pancreatic adenocarcinoma cell line SP2/0 myeloma cells.

Origin Pub PMID: 8519646

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: Q99795

Specificity Statement: hA33 binds specifically to the ectodomain of A33 cell-surface differentation antigen. The antigen is a single-pass type-1 membrane glyoprotein of the immunoglobulin superfamily and, while not present in most normal and tumor cell type, it is expressed on the vast majority (95%) of both primary and metastatic colon cancer cells. The protein is involved in cell-cell recognition and signaling.

Application Notes (Clone): The binding specificity and affinity of the humanized antibody has been shown to be equal to that of the orginal mouse antibody (1.3 nM), as is the uptake of 90Y-labelled antibody by human coloractal xenografts in nude mice. The circulating half-life of huA33 is also greater than that of the mouse antibody. Antigen-binding assays have been conducted by ELISA using Colo205 and SWI222 A33 antigen-expressing cells. Biodistribution assays have also been performed using hA33-12N4 conjugate in nude mice bearing subcutaneous SWI222 tumour xenografts. This antibody can be used as a biomarker for colorectal carcinoma.TFM (trivalent Fab fragment - constructed using trimaleimide cross-linkers) was shown to have a greater binding avidity for the target compared to huA33 with faster blood clearance shown in cynomolgus monkeys and guinea pigs.

• Artikelnummer: ABAAb00782-10.3
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab00782-10.3
• Verpackungseinheit: 200 μg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Recombinant
• Methode: Immunofluorescence, ELISA, Immunohistochemistry
• Isotyp: IgG1 kappa
• Wirt: Human