Anti-Acetylaminofluorene (K16-16)

Anti-Acetylaminofluorene [K16-16], Recombinant, Fab fragment kappa, Mouse

CloneID: K16-16

Heavy Chain modification: His-Tagged

Antigen Long Description: K16-16 mAb was generated by immnizing BALB/c mice with acetyl aminofluorene-substituted guanosine (Le Guernet al, 1984). RNA derived from this hybridoma clone was then used to construct a phage display library and was selected for by its affinity for AAF-labelled sonicated salmon sperm DNA in an ELISA screen.

Buffer Composition: PBS only.

Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv (IgG1) format, for improved compatibility with existing reagents, assays and techniques.

Specificity Statement: K16-16 binds to AAF-labelled DNA. AAF is used to label DNA and RNA by binding covalently at the C8 position of guanosines; AAF can then be detected using anti-AAF antibodies.

Application Notes (Clone): K16-16 was used to construct a recombinant conjugate by fusing PhoAv bacterial phosphatase. The conjugate was used for one-step immunodetection of DNAs and RNAs in dot-blot, colony and plaque hybridization and in Southern/northern blot hybridization. It has also been used to detect PCR products.

• Artikelnummer: ABAAb00759-1.6-BT
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab00759-1.6-BT
• Verpackungseinheit: 500 μg
• Mengeneinheit: STK
• Klonalität: Recombinant
• Methode: Dot Blot, Polymerase Chain Reaction (PCR), Hybridization
• Isotyp: Fab fragment kappa
• Wirt: Mouse