Anti-Acetylaminofluorene (K16-16)

Anti-Acetylaminofluorene [K16-16], Recombinant, IgG kappa, Rabbit
Artikelnummer
ABAAb00759-23.0
Verpackungseinheit
200 μg
Hersteller
Absolute Antibody

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CloneID: K16-16

Antigen Long Description: K16-16 mAb was generated by immnizing BALB/c mice with acetyl aminofluorene-substituted guanosine (Le Guernet al, 1984). RNA derived from this hybridoma clone was then used to construct a phage display library and was selected for by its affinity for AAF-labelled sonicated salmon sperm DNA in an ELISA screen.

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv (IgG1) format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Specificity Statement: K16-16 binds to AAF-labelled DNA. AAF is used to label DNA and RNA by binding covalently at the C8 position of guanosines; AAF can then be detected using anti-AAF antibodies.

Application Notes (Clone): K16-16 was used to construct a recombinant conjugate by fusing PhoAv bacterial phosphatase. The conjugate was used for one-step immunodetection of DNAs and RNAs in dot-blot, colony and plaque hybridization and in Southern/northern blot hybridization. It has also been used to detect PCR products.
Mehr Informationen
Artikelnummer ABAAb00759-23.0
Hersteller Absolute Antibody
Hersteller Artikelnummer Ab00759-23.0
Verpackungseinheit 200 μg
Mengeneinheit STK
Klonalität Recombinant
Methode Dot Blot, Polymerase Chain Reaction (PCR), Hybridization
Isotyp IgG kappa
Wirt Rabbit
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