CloneID: 5F6
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with an acrolein-treated keyhole limpet hemocyanin (KLH).
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for acrolein-modified proteins. Protein modification by acrolein is largely attributed to the formation of acrolein-lysine and acrolein-histidine adducts that possess a carbonyl function. While the antibody has been raised against acrolein-modified KLH, it has been shown to recognize both acrolein-modified BSA and oxidized LDL.
Application Notes (Clone): A direct ELISA examined the immunoreactivity of this antibody to aldehyde-treated proteins. It was scarcely inhibited by Na-acetyl-Nim-propanal-histidine and significantly inhibited by Na-acetyl-FDP-lysine, indicating that FDP-lysine (Nε-(3-formyl-3,4-dehydropiperidino)lysine) is an epitope. This antibody was also used for immunohistochemistry (IHC) detection of protein-bound acrolein in fatty streak lesions of arterial tissue (Uchida et al., 1998; PMID: 9560197). This antibody was used in flow cytometry (FC) to detect protein-bound acrolein in activated human T cells (Fonseca et al., 2001; PMID: 11342443). A novel pyridinium-type lysine adduct, Nϵ-(3-methyl-pyridinium)lysine (MP-lysine), was investigated and found to be a major epitope of this antibody (Furuhata et al., 2003; PMID: 14504272). This antibody was used for IHC to detect oxidative stress in the testis of rats (Iuchi et al., 2004; PMID: 14673659). This antibody was used for WB to detect acrolein modification of apoE3-NT (Tamamizu-Kato et al., 2007; PMID: 17580963).
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