CloneID: 2E8
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with human apoE3.
Origin Pub PMID: 8558079
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P02649
Specificity Statement: This antibody binds the LDL binding site 'RLASHLRKLRKRLLR' of the human apolipoprotein E. This antibody binds well with apoE3 and poorly with apoE2. Apolipoprotein E (apoE), a 299 amino acid lipoprotein, is a ligand for the low density lipoprotein receptor (LDLr). APO E is involved in the metabolism of fats and it is implicated in the Alzheimer's disease and cardiovascular diseases. It mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids.
Application Notes (Clone): This antibody was reported to binds to apoE and blocks apoE-mediated binding of lipoproteins to the low density lipoprotein receptor (LDLr). It was also reported that this antibody resembles LDLr in showing reduced reactivity against apoE (Arg158Cys). The reactivity of this antibody to various apoE variants was determined using ELISA (PMID: 8558079). This antibody partially blocked the binding of apoE isoforms to the immobilized VLDL receptor (PMID: 15863833).
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