CloneID: S2
Antigen Long Description: The original antibody was generated by immunizing female white leghorn chickens with 50 µg of recombinant B7-H4 protein (Phe29 to Ala258) through intramuscular injection. The spleens of the chickens were harvested 7 days after the final immunization to construct an scFv antibody library. The antibody was isolated by panning against B7-H4 protein.
Origin Pub PMID: 33716253
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length chimeric human antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q7Z7D3
Specificity Statement: This antibody binds a linear epitope comprising amino acids (VIQWLKEGVLGLVHEFK) of the human B7-H4.
Application Notes (Clone): This antibody recognized intact wild type human B7-H4 and linear epitope comprising amino acids 'VIQWLKEGVLGLVHEFK' in an ELISA. This antibody was also capable or recognizing B7-H4 in a western blot assay. The binding of this antibody to B7-H4 protein can effectively neutralize the immunosuppression response induced by B7-H4 proteins, thus enabling T-cells to retain their activation status (PMID: 33716253).
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