CloneID: 3C10
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q07812
Specificity Statement: The antibody is specific for Bax. It recognizes a peptide encompassing residues 31–45 at the start of Bax a1–a2 loop (AAs RAGRM).
Application Notes (Clone): The specificity of the antibody for Bax was determined by ELISA analysis. The antibody was able to bind to mitochondrial Bax and WT Bax by western blot analysis. The antibody immunoprecipitated Bax (Iyer et al., 2016; PMID: 27217060). The crystal structure of the Fab fragment in complex with BAX was determined. The antibody activates mitochondrial Bax, but blocks translocation of cytosolic Bax (Robin et al., 2018; PMID: 30122452).
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