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Application: Binding to BCMA by ELISA.
Assay Conditions: A plate was coated with Anti-BCMA Single-Chain Variable Fragment, His-Tag Molecule (#100173) at 100 ng/well. The plate was then incubated with BCMA, Fc-fusion (IgG1), Avi-Tag, Biotin-Labeled Recombinant (#79467) at different concentrations (0-300 nM in a 3-fold dilution series). Binding was detected with Strep-HRP.
Background: B-cell maturation antigen (BCMA), also known as CD269 or tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a cell surface receptor of the TNF receptor superfamily that recognizes B-cell activating factor (BAFF). BCMA is preferentially expressed in mature B lymphocytes and also on Multiple Myeloma (MM) cells. BCMA is a highly attractive target antigen for immunotherapy because of its restricted expression in non-malignant tissue but almost universal expression on MM cells. CAR (chimeric antigen receptor)-T BCMA cells are an autologous T cell product engineered by lentiviral transduction to express a fully human BCMA-specific CAR. CAR-T cells targeting BCMA have pre-clinical anti-MM activity, and in 2017, the FDA granted BCMA CAR T-Cell immunotherapy breakthrough designation in Multiple Myeloma.
Cross Reactivity: This antibody not been tested for cross-reactivity with other species.
Description: This molecule is a single-chain variable fragment (scFv) of an anti-BCMA (B-cell maturation antigen) antibody. This construct contains a C-terminal His-Tag (6xHis). This protein was affinity purified.
Format: Aqueous solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Purification: Ni-NTA affinity chromatography from HEK293 supernatants.
Purity: ≥85%
Storage Stability: Stable for at least 6 months at -80°C.
Tags: C-terminal His-tag
Target: BCMA
Uniprot: Q02223
Warnings: Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.
Biosafety Level: Not applicable (BSL-1)
References: 1. Schiemann, B., et al. Science. 2001. 293(5537): 2111-2114.2. Gross, J., et al. Nature. 2000. 404:995999.
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