Anti-Beta Lactamase (cAbTEM13)

Anti-Beta Lactamase [cAbTEM13], Recombinant, IgG1-Fc Fusion, Mouse

CloneID: cAbTEM13

Antigen Long Description: The original antibody was generated by immunizing adult dromedary (Camelus dromedarius) with TEM-1 β-lactamase.

Origin Pub PMID: 11557473

Buffer Composition: PBS only.

Uniprot Accession No.: P62593

Specificity Statement: This antibody binds an enzyme called beta-lactamase produced by bacteria, which catalysis the breaking of the beta-lactam ring of beta-lactam antibiotics such as penicillin, cephalosporin, cephamycin and monobactam through hydrolysis. The breaking of the beta-lactam ring deactivates the antibacterial properties of the antibiotic molecule and provides antibiotic resistance in bacteria.

Application Notes (Clone): The binding reactivity of this antibody to TEM-1 beta-lactamase was determined using ELISA. This antibody was capable of inhibiting beta-lactamase activity in vitro with an IC50 of 0.08 μM. The in vivo inhibition activity of this antibody to inhibit surface-exposed TEM-1 and prevent bacterial growth was assessed by preincubating cells expressing TEM-1 with increasing amounts of the antibody fragment. It was reported that when cells were preincubated with cAbTem13 before plating lost their ability to neutralize the antibiotic ampicillin (PMID: 11557473).

• Artikelnummer: ABAAb03503-1.159-BS
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab03503-1.159-BS
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Reaktivität: Escherichia Coli
• Klonalität: Recombinant
• Methode: ELISA
• Isotyp: IgG1-Fc Fusion
• Wirt: Mouse