CloneID: HBp2
Antigen Long Description: The original antibody was raised from splenic lymphocytes isolated from fresh human spleen tissue, that were immunized in vitro against Bordetella pertussis lipooligosaccharide.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for B. pertussis strains 9340 and Tohama 1, but not other strains
Application Notes (Clone): The original antibody was used for a dot blot on different bacteria lysates. This antibody was used for a radioimmunoassay on live bacteria, this antibody bound to surface-exposed epitopes on B. pertussis strains 9340 and Tohama 1, but not other strains. The biological activity of this antibody against B. pertussis and B. bronchiseptica was assessed. It showed a high degree of killing on B. pertussis strain 9340. This antibody was used for an ELISA on B. pertussis OMP preparations (Brodeur et al., 1991; PMID:1721552).