Anti-Butyrylcholinesterase  (11D8)

Anti-Butyrylcholinesterase  [11D8], Recombinant, IgG1 kappa, Human

CloneID: 11D8

Heavy Chain modification: Fc Silent™

Antigen Long Description: 11D8 wsa prepared by immunizing BChE -/- mice with sugar-diminished full-length recombinant human BChE (Hrabovska et al. 2010).

Origin Pub PMID: 26343001

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P06276

Specificity Statement: 11D8 binds to both native and denatured human butyrylcholinesterase (HuBChE) with KD ~0.26 nM (determined by ELISA - 0.9 nM by SPR). Binding of 11D8 excludes binding of mAb B2 18-5, suggesting tht that bind to the same/similar epitope on huBChE. The antibody also binds to human albumin, which can compete with huBChE for binding. huBChE in plasma is a serine esterase that catalyzes the hydrolysis of esters including aspirin, cocaine, heroin, and octanoyl ghrelin.

Application Notes (Clone): The percent active HuBChE bound to 11D8 in ELISAS is highest when the volume of plasma was lowest. 11D8 also binds to human albumin and so 11D8 is less suitable for immunopurification of huBChE from plasma compared to e.g. B2 18-5.

• Artikelnummer: ABAAb00765-10.3
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab00765-10.3
• Verpackungseinheit: 200 μg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Recombinant
• Methode: Immunoprecipitation, Western Blotting, ELISA, Immunohistochemistry
• Isotyp: IgG1 kappa
• Wirt: Human