CloneID: P7F4
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was generated by immunizing mice with various antigen preparations of B. pseudomallei (ATCC 23343). Later on phage display technique was used to generate scFv version of this antibody.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted mouse scFv antibody, based on the original mouse scFv format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds surface capsular antigens of Burkholderia pseudomallei (BP), a causative agent of melioidosis, is a gram-negative, facultative anaerobic, motile bacillus commonly found in the soil and stagnant waters. BP infection is often due to either direct inoculation into wounds and skin abrasions or inhalation of contaminated materials. Recently, BP has been recognized as a major cause of community-acquired septicemia, resulting in significant mortality. This antibody also cross reacts with surface capsular antigens of Burkholderia mallei. B. mallei (BM), a host-adapted pathogen that does not normally persist in nature, causes glanders in horse. Some studies indicated that BM is highly infectious in humans by aerosol route.
Application Notes (Clone): This antibody can be used for the detection of capsular antigens of Burkholderia pseudomallei and Burkholderia mallei in a sample using. The binding affinity of the antibody against B. pseudomallei was measured (0.449 at OD405nm) (Kim et al., 2011; PMID: 21172353).
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