CloneID: 5D3
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with hapten-carrier (BFNB-bovine serum albumin, BFNB-BSA) conjugates. BFNB stands for 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy) carbonyl]-amino]-butanoic acid.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds carbofuran, which is a carbamate pesticide used to control insects on a wide variety of field crops, including potatoes, corn and soybeans. It is a synthetic insecticide which is absorbed by the plants such that insecticidal concentration can be attained.
Application Notes (Clone): The binding affinity and the specificity of this antibody were characterized by ELISA or indirect competitive ELISA (Yang et al., 2007). This antibody was also used to develop a rapid, specific and sensitive ELISA method to determine residues of the pesticide carbofuran in a variety of matrices (PMID: 18463589).
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