CloneID: AB1
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: O89093
Specificity Statement: The antibody is specific for murine CCL20. The antibody does not recognise human CCL20.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. Capture ELISA was performed and the 1:1 binding kinetics and affinities were measured by surface plasmon resonance (Kd= 2.3 nM). CCR6 Chinese hamster ovary (CHO)-K1 β-arrestin G protein–coupled receptor functional assay was performed to measure the inhibition of the antibody (IC50= 30.7).
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